Poster Title

Evidence for lack of expression of Cytochrome P450 1A1 in paddlefish

Poster Number

013

First Faculty Mentor

Dr. Jeannine M. Chan

Department

chemistry

Poster Abstract

The induction of cytochrome P450 1A1 (CYP1A1) in fish is a classic biomarker for a polluted aquatic ecosystem. In 2000, Gunderson et al. found no indication of CYP1A1 induction in paddlefish of the Ohio River water system, though a number of pollutants known to induce CYP1A1 expression were present in their aquatic habitat. In the present study, samples of hatchery paddlefish, known to be exposed to minimal pollutants, as well as hatchery paddlefish injected with β-naphthoflavone, a known inducer of CYP1A1 in other fish species, were tested for CYP1A1 activity using a 7-ethoxy-o-deethylase (EROD) assay. Presence of the enzyme was also assessed using Western blot analysis. Consistent with the prior study, no EROD activity was found in any of the paddlefish samples, suggesting that CYP1A1 in paddlefish is not a viable option for a biomarker in the Ohio River.

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Nov 16th, 12:00 AM

Evidence for lack of expression of Cytochrome P450 1A1 in paddlefish

The induction of cytochrome P450 1A1 (CYP1A1) in fish is a classic biomarker for a polluted aquatic ecosystem. In 2000, Gunderson et al. found no indication of CYP1A1 induction in paddlefish of the Ohio River water system, though a number of pollutants known to induce CYP1A1 expression were present in their aquatic habitat. In the present study, samples of hatchery paddlefish, known to be exposed to minimal pollutants, as well as hatchery paddlefish injected with β-naphthoflavone, a known inducer of CYP1A1 in other fish species, were tested for CYP1A1 activity using a 7-ethoxy-o-deethylase (EROD) assay. Presence of the enzyme was also assessed using Western blot analysis. Consistent with the prior study, no EROD activity was found in any of the paddlefish samples, suggesting that CYP1A1 in paddlefish is not a viable option for a biomarker in the Ohio River.